Sunday, February 9, 2025
Symbiosis: Nature’s Collaborative Masterpiece
Monday, July 30, 2018
ATP- biological currency
(https://www.britannica.com/science/cellular-crespiration)
Have you every found yourself completely drained of every ounce of energy? You needed a healthy snack.
We can stay energized and keep our bodies energized. Cellular respiration yields ATP.
Cellular respiration is important because it provides the energy for living organisms to perform all of the other necessary functions to maintain life. Our energy currency is ATP.
How is ATP made?
ATP is composed of Adenosine tri-phosphate-
a compound consisting of an adenosine molecule bonded to three phosphate groups, present in all living tissue. The breakage of one phosphate linkage (to form adenosine diphosphate, ADP ) provides energy for physiological processes such as muscular contraction.
ATP is composed of Adenosine-
Adenosine is a nucleoside that is composed of Ademine and D-ribose. Adenosine derivatives play many important biological roles in addition to being components of DNA and RNA. Adenosine is a neurotransmitter.
ATP contain Phosphate. A phosphate can be grouped as any of numerous chemical compounds related to phosphoric acid (H3PO4). One group of these derivatives is composed of salts containing the phosphate ion (PO43−), the hydrogen phosphate ion (HPO42−), or the dihydrogen phosphate ion (H2PO4−), and positively charged ions such as those of sodium or calcium. A second group is composed of esters, in which the hydrogen atoms of phosphoric acid have been replaced by organic combining groups such as ethyl (C2H5), or phenyl (C6H5).
Energy is formed through an ATP reaction.
ATP Hydrolysis-
Adenosine triphosphate is composed of the nitrogenous base adenine, the five-carbon sugar ribose, and three phosphate groups.ATP is hydrolyzed to ADP in the reaction ATP+H2O→ADP+Pi+ free energy. The calculated ∆G for the hydrolysis of 1 mole of ATP is -57 kJ/mol.ADP is combined with a phosphate to form ATP in the reaction ADP+Pi+free energy→ATP+H2O.The energy released from the hydrolysis of ATP into ADP is used to perform cellular work, usually by coupling the exergonic reaction of ATP hydrolysis with endergonic reactions.Sodium-potassium pumps use the energy derived from exergonic ATP hydrolysis to pump sodium and potassium ions across the cell membrane while phosphorylation drives the endergonic reaction.
Cellular respiration is supported by several key reactions:
Tricarboxylic acid cycle
Glycolysis
Aerobic oxidation
Glyoxylate cycle
Reductive tricarboxylic acid cycle
Pasteur effect
Energy flow
Catabolism
The Human body goes through thousands of bodily processes, a day.
Cellular respiration is vital. This biological pathway provides the energy for humanity to perform all of the necessary functions to cellular life.
Our energy currency is ATP.
Tuesday, April 17, 2018
Diphenylamine, apple pesticide
Go to your nearest edible supplier. Pick up an apple. What is the powder substance on the sides? It is a pesticide, which slows down produce mold. A popular pesticide is diphenylamine.
(Information origination-
Reregistration Eligibility Decision (RED) document fact sheet case 2210, United States Prevention, Pesticides EPA-738-F-97-010 Environmental Protection And Toxic Substances April 1998 Agency (7508W))
Diphenylamine (DPA) is a plant growth regulator used post-harvest to control storage scald on apples. It is mainly used for its antioxidant properties. As a pesticide, DPA is used for destroying insects or other organisms harmful to cultivated plants. DPA has been classified as an indoor food use.
Storage scald- term loosely applied to a group of skin disorders of apples and pears. It involves brown or gray discoloration of irregularly shaped areas on the surface of the fruit during or following storage (http://postharvest.tfrec.wsu.edu/pages/N6I2C)
Chemical formula- C12H11N
Molar Mass- 169.23 g/mol
Appearance- White, tan, amber, or brown crystals
Odor- pleasant, floral
Density- 1.2 g/cm3
Melting point- 53 °C (127 °F; 326 K)
Boiling point- 302 °C (576 °F; 575 K)
Solubility in water- 0.03%
Vapor pressure- 1 mmHg (108°C)
Acidity (pka)- 0.79
Magnetic susceptibility- -109.7·10−6 cm3/mol
Formulations include an emulsifiable concentrate, a wettable powder, a soluble concentrate/liquid and a ready-to-use liquid. DPA is applied by dipping, drenching or spraying. It was pesticide registered in 1947.
DPA has been shown to be slightly toxic by the oral, dermal, and inhalation routes and has been placed in Toxicity Category III (second lowest of four categories) for these effects. The dietary intake cancer risk for diphenylnitrosamine is 2.8 x 10 mg/kg/day.
DPA is of low acute toxicity and has been classified as “Not Likely“ as a human carcinogen. An impurity of DPA,
diphenylnitrosamine, is classified as a “probable human carcinogen”, however the cancer risks for dietary intake and worker exposure fall below the Agency’s level of concern. Food crop use consist of post-harvest use on apples. Dietary exposure to DPA residues in foods is within acceptable limits.
Since this is considered an indoor food end-use chemical, only hydrolysis data are required. Available data indicate that DPA is stable towards hydrolysis at pH’s 5, 7, and 9. DPA has a moderate solubility in water (39.4 ppm), a relatively high octanol/water partition coefficient (K =3,860), and a high vapor pressure (6.39 x 10 torr). The high rate of ow
-4 aqueous photolysis and the susceptibility of the chemical in aerobic environments indicate that if DPA were to reach surface waters, it would be short lived.
DPA is moderately toxic to fish and aquatic invertebrates. It is practically non-toxic to avian species on an acute and subacute basis.
Industrial handling of Diphenylamine-
The Agency develops any mitigation measures or regulatory controls needed to effectively reduce each pesticide's risks. EPA then reregisters pesticides that meet the safety standard of the FQPA and can be used without posing unreasonable risks to human health or the environment. When a pesticide is eligible for reregistration, EPA explains the basis for its decision in a Reregistration Eligibility Decision (RED) document. These facts are summarized from the RED document for reregistration case 2210, diphenylamine.
Friday, January 26, 2018
Zika Virus update
1. Effective-
In both cell cultures and mouse models, a drug used to treat Hepatitis C effectively protected and rescued neural cells infected by the Zika virus -- and blocked transmission of the virus to mouse fetuses.
Cell culture-
refers to the removal of cells from an animal or plant and their subsequent growth in a favorable artificial environment.
Mouse models-
mice naturally develop conditions that mimic human disease conditions, so inbred laboratory mice have been used as model organisms to study the virus.
2. Useage-
Researcherssay their findings support further investigation of using the repurposed drug as a potential treatment for Zika-infected adults, including pregnant women.
3. Explanation-
Research took place in the Muotri Lab, University of California San Diego, 9500 Gilman Dr, La Jolla, CA 92093.
Goal: a cure for the zika virus.
"The team investigated an antiviral drug called sofosbuvir, approved and marketed under the brand name Sovaldi to treat and cure hepatitis C infections. The drug works by inhibiting replication of the hepatitis C virus. Researchers noted that both hepatitis C and Zika belong to the same viral family and bore strong structural similarities that could make sofosbuvir effective against the latter. In addition, it had been reported that sofosbuvir was protective against Zika in different cell types (https://www.sciencedaily.com/releases/2018/01/180125135529.htm)."
Zika vs. Sofosbuvir-
a. rescued dying NPCs infected with the Zika
b. restored gene expression linked to their antiviral response
c. arrested Zika replication in vivo
d. stopped flow from mother to fetus
e. the drug was well-tolerated by the Zika-infected pregnant mice
"In subsequent tests using an immunodeficient mouse model infected by Zika, intravenous injections of sofosbuvir significantly reduced viral loads in blood serum compared to a placebo group. Moreover, fetuses of Zika-infected pregnant mice did not show detectable Zika virus amplification in the sofosbuvir-treated group."
http://ucsdnews.ucsd.edu/pressrelease/repurposed_drug_found_to_be_effective_against_zika_virus
More research needs to be done, but we are approaching the effective cure of the Zika virus.
Tuesday, August 22, 2017
Innate Immunity and Immune System Infection
The immune system is our internal defense.
There are many potentially dangerous viruses, bacteria, and other pathogens. They occur in the air, in the food, and in the water. There are also cells that abnormally develop into cancer. Our immune system has developed two types of defense against these threats. They are innate immunity and acquired immunity.
Let's look at innate immunity.
Innate immunity is the kind of defense that is mediated by phagocytic (uptake) cells, antimicrobial proteins, the inflammatory response, and natural killer (eliminates tumors and viruses) cells. It is present before exposure to pathogens and is effective from the time of birth.
The first lines of defense are the external defenses. Intact skin and mucous membranes form physical barriers that bar the entry of microorganisms and viruses. Mucus produced by cells in these membranes, the low pH (acidic ranging from 3-5) of the skin and stomach, and degradation by lysozyme (emzyme that digests bacterial cell walls) also deter infection by pathogens.
The second lines of defenses are the internal cellular and chemical defenses. Phagocytic cells (white blood cells) injest microbes that penetrate external innate defenses and help trigger an inflammatory response. Phagocytes attach to their prey via surface receptors that bind to certain structures found on many microorganisms, but not on normal body cells. Complement proteins, interferons, and other antimicrobial proteins also act against invading microbes. In local inflammation, histamine and other chemicals released from injured cells promote changes in blood vessels that allow fluid, more phagocytes, and antimicrobial proteins to enter the tissues. Although heat and swelling are uncomfortable sensations, the enhanced blood flow and vessel permeability that cause them are critical to innate defense. Natural killer cells induce the death of virus-infected cancer cells via apoptosis (programmed cell death).
Invertebrate immune mechanisms are present, also. Insects defend themselves by mechanisms similar in many respects to vertebrate innate defenses. The insect equivalent to blood, the hemolymph, contains circulating cells called hemocytes. Antimicrobial peptides and phenolooxidase are insect innate immune defenses.
Defenses in vertebrates can be divided into innate and acquired immunity. If an invading pathogen reaches the body's external innate defenses, various internal innate defenses quickly come into play. The defenses provided by acquired immunity against specific pathogens develop more slowly. Some components of innate immunity also function in acquired immunity.
Wednesday, April 5, 2017
The Cell Cycle
Cell cycle- (http://www.medicinenet.com/script/main/mobileart.asp?articlekey=7107)
A cell is the basic unit of life.
How do we make new cells?
A cell is a microscopic structure containing nuclear and cytoplasmic material enclosed by a semipermeable membrane and, in plants, a cell wall; the basic structural unit of all organisms. We have prokaryotic cells and eukaryotic cells.
A prokaryotic cell possesses a simple structure with no nuclear envelope and usually a single circular chromosome. A eukaryotic cell possesses a more complex structure, with a nucleus and multiple linear chromosomes consisting of DNA complexed to histone proteins.
Cell reproduction requires a copy of the genetic material, separation of the copy, and cell division. In a prokaryotic cell, the single chromosome replicates, each copy moves toward opposite sides of the cell, and the cell divides. In eukaryotic cells, reproduction requires mitosis and meiosis to ensure that a complete set of genetic information is transferred to each new cell. In eukaryotic cells, chromosomes are typically found in homologous pairs.
Functional chromosomes consist of a centromeres, telomeres, and multiple origins of replication. The centromere is the point at which the kinetochore assembles and to which microtubules attach. Telomeres are the stable ends of chromosomes. After a chromosome is copied, the two copies remain attached to the centromere, forming sister chromatids.
The cell cycle consists of the stages through which a eukaryotic cell passes between cell divisions. It consists of: G-subzero, Interphase, G-subone, A-phase, G-subtwo, M-phase, Prophase, Prometaphase, Metaphase, Anaphase, Telophase, and Cytokinesis.
"The cell cycle governs the entire life of a cell, including growth, division and death. There are defined phases of the cell cycle, during which the cell grows, duplicates its DNA and eventually divides into two new cells (http://sciencing.com/cell-cycle-20206.html)."
At the end of the cell cycle the chromosomes line up in the center of the cell. Sister chromatids separate and become independent chromosomes, which then migrate to opposite ends of the cell. The nuclear membrane reforms around chromosomes at each end of the cell, and the cytoplasm divides.
Mitosis results in the production of two genetically identical cells. Progression through the cell cycle is controlled by interactions between cyclins and cyclin-dependent kinases.
Sexual reproduction produces genetically variable progeny and allows for accelerated evolution. It includes meiosis, in which haploid sex cells are produced, and fertilization, the fusion of sex cells.
The major events of meiosis include: Meiosis one, Prophase one, Metaphase one, Anaphase one, Telophase one, Cytokinesis, Meiosis two, Prophase two, Metaphase two, Anaphase two, Telophase two, and Cytokinesis.
Genetic variation in meiosis is produced by crossing over and by the random distribution of maternal and paternal chromosomes. The result of meiosis is the production of four haploid cells that are genetically variable.
"Genetic variation can refer to differences between individuals or to differences between populations. Mutation is the ultimate source of genetic variation, but mechanisms such as sexual reproduction and genetic drift contribute to it as well (https://geneed.nlm.nih.gov/topic_subtopic.php?tid=48&sid=50)."
Grasping mitosis and meiosis requires more than simply memorizing the sequence events that take place in each stage, although these events are important. The key is to understand how genetic information is apportioned in the course of cell reproduction through a dynamic interplay of DNA synthesis, chromosome movement, and cell division. These processes bring about the transmission of genetic information and are the basis of similarities and differences between parents and progeny.


